cloning of glutamate decarboxylase and production of γ-aminobutyric acid from lactobacillus brevis
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چکیده
منابع مشابه
Identification and molecular cloning of glutamate decarboxylase gene from Lactobacillus casei
Gamma-amino butyric acid (GABA) possesses several physiological functions such as neurotransmission, induction of hypotension, diuretic and tranquilizer effects. Production of GABA-enriched products by lactic acid bacteria has been a focus of different researches in recent years because of their safety and health-promoting specifities. In this study, glutamate decarboxylase (gad) gene of a loca...
متن کاملHydrogel Film-Immobilized Lactobacillus brevis RK03 for γ-Aminobutyric Acid Production
Hydrogels of 2-hydroxyethyl methacrylate/polyethylene glycol diacrylate (HEMA/PEGDA) have been extensively studied for their use in biomedical and pharmaceutical applications owing to their nontoxic and highly hydrophilic characteristics. Recently, cells immobilized by HEMA/PEGDA hydrogels have also been studied for enhanced production in fermentation. Hydrogel films of HEMA/PEGDA copolymer wer...
متن کاملGlutamate decarboxylase from Lactobacillus brevis: activation by ammonium sulfate.
In this study, the glutamate decarboxylase (GAD) gene from Lactobacillus brevis IFO12005 (Biosci. Biotechnol. Biochem., 61, 1168-1171 (1997)), was cloned and expressed. The deduced amino acid sequence showed 99.6% and 53.1% identity with GAD of L. brevis ATCC367 and L. lactis respectively. The His-tagged recombinant GAD showed an optimum pH of 4.5-5.0, and 54 kDa on SDS-PAGE. The GAD activity a...
متن کاملmolecular gene cloning and sequencing of glutamate decarboxylase gene from lactobacillus delbrueckii and lactobacillus reuteri
glutamate decarboxylase enzyme produces γ-aminobutyric acid (gaba) in a non-reversible decarboxylation reaction of glutamate. gaba is a major inhibitory neurotransmitter of the brain and it is also present at high concentration in other organs such as pancreatic islets. gaba has effects on blood pressure, diabetes, inflammation, sleeplessness and depression. some bacteria such as lactobacillus ...
متن کاملCharacterization of a Potential Probiotic Lactobacillus brevis RK03 and Efficient Production of γ-Aminobutyric Acid in Batch Fermentation
Lactic acid bacteria were isolated from fish and evaluated for their γ-aminobutyric acid (GABA)-producing abilities. Out of thirty-two isolates, Lactobacillus brevis RK03 showed the highest GABA production ability. The effects of various fermentation parameters including initial glutamic acid level, culture temperature, initial pH, and incubation time on GABA production were investigated via a ...
متن کاملPartial Cloning and Nucleotide Sequencing of Glutamate Decarboxylase Gene Isoform 65 from Human Brain
Background: Gamma -aminobutyric acid (GABA), a non-protein amino acid acts as an inhibitory neurotransmitter in the central nervous system of mammalians. The glutamate decarboxylase (GAD) is responsible for the conversion of L-glutamate to GABA. The human brain has two isoforms of this enzyme, GAD65 and GAD67 that differ in molecular weight, amino acid sequence, antigenicity, cellular location ...
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عنوان ژورنال:
research in pharmaceutical sciencesجلد ۷، شماره ۵، صفحات ۰-۰
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